Extended Data Fig. 3: BMP signalling is required for strut formation.
From: Endothelial struts enable the generation of large lumenized blood vessels de novo
a, Mosaic expression of laClover under the control of the EC specific promoter fli1a. Arrowhead indicates mClover positive strut at 22 hpf (upper panel). Lower panel shows an image of the same region at 48 hpf. Images are representative of 9 embryos analysed from three independent experiments. b, Gating strategy used to FACS sort ECs from dissected CV regions followed by qPCR (10 CVs were pooled per condition measured in two independent experiments). Of note, the bmp pathway is active in multiple tissues at this time, including erythrocytes and strong activity in epithelial cells (Fig. 3e asterisks), these cells are included in the unsorted CV (black bars). Scale bars are defined in the figure.
