Extended Data Fig. 4: Inhibition of blood flow prevents struts from pruning.
From: Endothelial struts enable the generation of large lumenized blood vessels de novo
a, Gating strategy used to FACS sort ECs from dissected CV regions followed by qPCR (b) The onset of blood flow was inhibited by either injecting of a cardiac troponin T2a (tnnt2a) MO or through administration of 3x concentrated ms-222 (tricaine methanesulfonate). Erythrocytes are marked by GFP (globin) and all ECs by laCherry (magenta). Data are presented as the median values with the first and third quartiles; the whiskers represent the minimum and maximum. One-way analysis of variance (ANOVA) with Dunn’s post-hoc test. ns = not significant (n = 25 embryos per time points per experiment of three independent experiments). Images are representative of 5 embryos analysed by confocal microscopy per condition per experiment. c, 48 hpf embryo injected with tnnt2a MO. Asterisks indicate an incomplete formation of the CV wall. Images are representative of 10 embryos analysed from two independent experiments. Scale bars are defined in the figure.
