Extended Data Fig. 2: The FGFR/Btl is necessary and sufficient for midgut TTC branching and ISC mitosis.
a-b, Brightfield images of the tracheae of P.a. infected R5 regions of the midgut in trh-Gal4 control (a) and trh-Gal4 > UAS-btlDN (b). c-d, Brightfield images of the tracheae of uninfected R5 regions of the midgut in trh-Gal4 control (c) and trh-Gal4 > UAS-λbtl (d). e-g, Quantification of TTCs (e, n = 10,11,10,9,10), TTC branching (f, n = 10,11,10,8,10), and midgut mitosis (g, n = 8,6,10,9,6) upon trh-Gal4-driven btl manipulation with or without P.a. infection. h-i, Brightfield images of the tracheae of P.a.-infected R5 regions of the midgut in dSRF-Gal4 control (h) and dSRF-Gal4 > UAS-btlDN (i). j-k, Brightfield images of the tracheae of uninfected R5 regions of the midgut in dSRF-Gal4 control (j) and dSRF-Gal4 > UAS-λbtl (k). l-n, Quantification of TTCs (l, n = 10,10,8,8,11), TTC branching (m, n = 10,10,8,8,11), and midgut mitosis (n, n = 11,9,12,12,9) upon dSRF-Gal4-driven btl manipulation with or without P.a. infection. All scale bars: 75 μm. Data are presented as mean values ± SD. Statistical significance (t-tested, two-sided): ns, not significant, * 0.01 < p ≤ 0.05, ** 0.001 < p ≤ 0.01 and *** p ≤ 0.001.
