Extended Data Fig. 4: Btl/Bnl signaling in the epithelial cells is necessary for efficient tracheal remodelling and mitosis in response to infection.

a-b, Quantification of TTC branching upon progenitor- (a) and EC-specific (b) silencing of bnl (bnl^RNAi3) and btl (btl^RNAi) (a, n = 10,8,5,10,7,7 and b, n = 10,7,9,10,9,8). c-d, Quantification of midgut mitosis upon progenitor- (c) and EC-specific (d) silencing of bnl (bnl^RNAi3) (c, n = 6,8,12,12 and d, n = 9,9,11,13). e, Quantification of esg + progenitors as a percent of total number of cells in the posterior regions of the midgut upon progenitor-specific knockdown of btl (btl^RNAi) and bnl (bnl^RNAi3) (n = 12,15,15). f-g, Quantification of midgut mitosis upon progenitor- (f) and EC-specific (g) silencing of btl (btl^RNAi) (f, n = 8,13,11,13 and g, n = 12,11,11,16). h, Quantification of esg + progenitor cells/total number of cells in the posterior midgut upon progenitor-specific knockdown of btl (btl^DN, n = 9,9). Data are presented as mean values ± SD. Statistical significance (t-tested, two-sided): ns, not significant, * 0.01 < p ≤ 0.05, ** 0.001 < p ≤ 0.01 and *** p ≤ 0.001.

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