Extended Data Fig. 7: Time-course analysis of NotchDN progenitor-derived midgut tumors.
a-d, The R4a region of control (reared for 4 days at 18 °C) (a) and tumorous midguts (reared for 4, 7 and 10 days at 29 °C) (b-d) of the esg-Gal4 UAS-eGFP tub-Gal80ts > UAS-NotchDN genotype with concomitant expression of QF6 > QUAS-mtdTomato (red) to label the trachea. DAPI (blue in a-d) is used to label all midgut nuclei and Prospero (a”-d”) labels the EEs. a’-d’, a”-d” and a”’-d”’ correspond to the individual channels for eGFP, Prospero and Tomato-labeled trachea, respectively. Scale bars: 75 μm. e-g, Quantification of TTC branching in the R4a of control (NotchDN uninduced) and NotchDN-expressing midguts (e, n = 4,8,4,6,4,7), in the NotchDN tumor-region vs. neighboring non-tumor area on the same image (f, n = 6,6,4,4,6,6), and midgut mitosis of control (NotchDN uninduced) and NotchDN-expressing midguts (g, n = 20 each) during a time-course analysis at 4, 7, and 10 days post-tumor induction. Scale bar: 75 μm. Data are presented as mean values ± SD. Statistical significance (t-tested, two-sided): ** 0.001 < p ≤ 0.01 and *** p ≤ 0.001.
