Extended Data Fig. 5: Schematic of the preferred sites of action for the different programmable RNA-targeting systems. | Nature Cell Biology

Extended Data Fig. 5: Schematic of the preferred sites of action for the different programmable RNA-targeting systems.

From: Non-invasive and high-throughput interrogation of exon-specific isoform expression

Extended Data Fig. 5

Alternative splicing (AS) generates different isoforms of a single pre-mRNA, regulated by splice factors. b, RNA-guided perturbation using RfxCas13d-NLS in the nucleus is expected to be isoform-unspecific but can be made isoform-specific by targeting the exon-exon-junctions. c, In contrast, cytosolic PspCas13b-NES acts in the cytosolic environment and can thereby degrade isoforms without the necessity for targeting the exon-exon-junctions. Endogenously reprogrammed microRNAs can also efficiently act in the cytosol. In comparison to PspCas13b-NES, a few restrictions on the choice of the RNA sequence apply. d, In the case of the inactivated variant dRfxCas13d-NLS, splice enhancers/suppressors can be recruited to an exon of interest to modulate exon in- or exclusion resulting in an isoform shift.

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