Extended Data Fig. 6: Validation of key results of isoform-specific knockdown of MAPT with RT-qPCR.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
a, Optimization of spacer length for RfxCas13d with a nuclear localization sequence (NLS) measured via depletion of induced tau, tracked via mRNA levels of 4R-tau and pan-tau 72 h post-transfection. b, Targeting of exon-exon junctions to increase isoform specificity of RfxCas13d-NLS. c, Comparison of isoform specificity in cytosolic targeting of mature mRNA with different amiRNAs. Data shown in a-c represent the mean values ± s.d. (n = 3 biological replicates). Selected results are shown for a two-way ANOVA with Bonferroni MCT (a,b), or two-tailed unpaired t-test between the two exon-10 targeting effectors (c), where* and *** denote p-values smaller than 0.05 and 0.001 (full statistical results are available in Supplementary Table 1). Numerical source data are provided in Source Data Extended Data Fig. 6.
