Extended Data Fig. 7: Mapping of potential off-target binding sites of RfxCas13d/PspCas13b crRNAs.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
The number indicated above the binding sites denotes the targeted exonic region of MAPT (for example, 10-11 is the intended targeting of the exon-junction 10-11). ‘x’ indicates mismatches on the isoform off-target sites, and ‘w’ indicates wobble base pairs (G-U pairings). 10-11 targeting amiRNA has 3 terminal mismatches, which have been shown to be well-tolerated in shRNAs, resulting in a depletion of both 4R and 3R tau isoforms. 29-10 crRNA (RfxCas13d-NLS) also matches the 9-11 junction resulting in an unspecific depletion of both 4R and 3R tau.
