Extended Data Fig. 10: Genomic DNA analysis of cell populations selected with increasing blasticidin S concentration after MBNL1/2-KO.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
Genomic DNA was isolated from cell populations selected with blasticidin at three different concentrations (top row to bottom row). The frequency of insertions and deletions were estimated for a, the MBNL1 or b, the MBNL2 locus from Sanger sequencing chromatograms. Cells resistant to a blasticidin S in a concentration-dependent manner showed an increased frequency in mutated MBNL1 alleles confirming the prominent role of MBNL1 in suppressing FOXP1 exon 18b inclusion. This analysis was conducted once.
