Fig. 3: hiPSC-derived EXSISERS lines for pharmacological screening.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
a, smNPCs were generated from hiPSCs into which EXSISERS was introduced into exon 10 and 11 (EXSISERSMAPT:10NLuc-11FLuc). b, WT and IVS10+16 C>T hiPSCs were differentiated into cortical neurons over three months. The Nluc/Fluc ratios are shown normalized to WT at day 0. c, The NLuc/FLuc ratios (4R/pan-tau) are plotted as a function of increasing concentrations of the DYRK1A/GSK3A inhibitor ITU in EXSISERSMAPT:10NLuc-11FLuc smNPCs after 24 h, 48 h and 72 h. For b and c, data are mean ± s.d., n = 3 biological replicates. Selected results of Bonferroni MCT after one-way ANOVA analysis are shown; *P < 0.05, **P < 0.01; ***P < 0.001, ****P < 0.0001 (full statistical results are provided in Supplementary Table 1). Source data are available online.
