Fig. 7: RNA helicase MOV10 is involved in FOXP1 exon 18b suppression.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
a, The reads (normalized to preselection reads) from both selection conditions (3 µg ml−1 and 5 µg ml−1 blasticidin S). The areas highlighted in light blue indicate the most strongly enriched sgRNAs for each selection condition. These data are replotted from Fig. 6c. b, The enrichment of MOV10 in the screen was confirmed by an independent MOV10-targeting sgRNA 2 (MOV10 2), which was not used in the screen. Representative colonies in a T75 flask are shown 2 weeks after selection with 3 µg ml−1 blasticidin S and after transfection with an sgRNA against ALDOA (unrelated control gene, <10 colonies in T75 flask), the MOV10-targeting sgRNA used in the screen (MOV10 1, >100 colonies in T75 flask), or MOV10 2 (>200 colonies in T75 flask). Scale bar, 1 mm. c, RT–PCR showing the blasticidin-S-concentration-dependent inclusion of FOXP1 exon 18b from the colonies surviving blasticidin S selection shown in b labelled with the respective sgRNAs. The data represent an independent validation of the results shown in a.
