Extended Data Fig. 1: Improving internal split-inteins with coiled-coils as heterodimerization domains to support excision of the reporter module.
From: Non-invasive and high-throughput interrogation of exon-specific isoform expression
The schematic on the top left panel depicts the construct containing split-inteins without (left) and with (right) addition of coiled-coils (yellow) flanked by the two halves of split-mNeonGreen (green rectangular shapes) serving as a model for exteins with extremely fast folding rates of less than 10 minutes. The immunoblot analysis of mNeonGreen (FLAG-tag, 24 hours post-transfection) showed a substantial increase of the resulting extein for the construct with the coiled-coils. Strong overexposure reveals a small fraction of side products (C-cleavage product C-mNeonGreen). Data shown represent 2 independent experiments. Unprocessed blots are provided in Source Data Extended Data Fig. 1.
