Extended Data Fig. 5: Global gene-expression changes in response to UV irradiation.

Volcano plots of RNA-seq in the indicated RPE1-iCas9 cell lines depicting the downregulation (blue) or upregulation (red) of gene expression in response to UV irradiation (24 h; 9 J m^-2). The fold change (log₂) is plotted on the x-axis and the significance (-log₁₀ P value) is plotted on the y-axis. a, WT, (b) CSB-KO, (c) ELOF1-KO. Only genes indicating at least 2 counts per million (CPM) in at least 33% of samples were included in the analysis. FDR-adjusted P values < 0.05 were considered significant. Two short UV-response genes (ATF3, CDKN1A) are highlighted. d, Box plot depicting the gene length of the 650 most significantly downregulated genes (in blue) or the 650 most significantly upregulated genes (in red). The horizontal line represents the median (center); Upper Bound: gene length scores larger than 75% of all data points; Lower Bound: gene lengths scores shorter than 75% of all data points. Points above and below the box represent the outliers.