Extended Data Fig. 1: Human ELOF1 protects cells against transcription stress.
From: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation
a, Front-view and side-view of the yeast orthologue of CSB, Saccharomyces cerevisiae RAD26 (purple), bound to RNAPII (grey) (PDB: 5VVS). b, Schematic representation of the CRISPR/Cas9 screen in RPE1-iCas9 cells in the presence of Illudin S (IC60; 25 nM). c, Network analysis of highly significant hits representing genes that promote Illudin S toxicity. Grey lines reflect known protein-protein interactions (Cytoscape, BioGRID). d, Sanger sequencing of the indicated RPE1-iCas9 single ELOF1-KO clones. e, 72 h drug sensitivity assay of indicated RPE1-iCas9 KO clones. The experiment has been performed twice and each symbol represents the median of 6 technical replicates of an independent experiment. UT, untreated f, Clonogenic Illudin S survival of the indicated RPE1-iCas9 cells. The experiment has been performed three times (except for ELOF1-KO 2-12 where the experiment has been done twice) and each symbol represents the mean of 2 technical replicates of an independent experiment. Numerical data are provided in Source data extended data Fig. 1.
