Extended Data Fig. 4: CCM3 regulates YAP/TAZ activation independently of the CCM complex. | Nature Cell Biology

Extended Data Fig. 4: CCM3 regulates YAP/TAZ activation independently of the CCM complex.

From: RETRACTED ARTICLE: CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling

Extended Data Fig. 4

a, Immunofluorescence analysis of YAP/TAZ in HN-CAFs, V-CAFs, and Cer-CAFs depleted of CCM1, CCM2 or CCM3 and stained for YAP/TAZ (green), F-actin (red), and DAPI (blue). The scatter plots show quantification of the YAP/TAZ nuclear/cytoplasmic ratio. Each data point represents individual cells from three independent experiments. n: individual cells (HN-CAF: siCtr, 24; siCCM1, 24; siCCM2, 25; siCCM3, 32. V-CAF: siCtr, 78; siCCM1, 60; siCCM2, 69; siCCM3, 83. Cer-CAF: siCtr, 34; siCCM1, 23; siCCM2, 26; siCCM3, 65). Line and error bars indicate mean ± s.d.; one-way ANOVA test. Scale bar, 50 μm. b, Loss of CCM3 induces YAP/TAZ translocation on soft matrices. Immunofluorescence analysis of YAP/TAZ localization in Cer-CAFs plated on collagen-coated softwells with increasing stiffness. Statistical data are provided in the source data.

Source data

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