Extended Data Fig. 1: Loss of CCM3 activates CAFs. | Nature Cell Biology

Extended Data Fig. 1: Loss of CCM3 activates CAFs.

From: RETRACTED ARTICLE: CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling

Extended Data Fig. 1

a, HN-CAFs and Cer-CAFs were stained for phospho-S19 myosin light chain 2 (pS19-MLC2 in green), F-actin (red), and DAPI (blue). Scale bars, 50 μm. b, Immunoblotting analyses of the contractile machinery in HN-CAFs and Cer-CAFs, 72 h after siRNA transfection. c, Knockdown efficiency of CCM3 and induction of α-SMA and pS19-MLC2 among all CCM3 siRNAs demonstrates reproducibility. d, Representative traction-force heat maps for transfected V-CAFs. Uncropped blots are provided in the source data.

Source data

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