Extended Data Fig. 3: Effective and specific labelling of perinatal ventricular endocardial cells in Npr3CreERT2.
From: Perinatal angiogenesis from pre-existing coronary vessels via DLL4–NOTCH1 signalling
a,b, Co-immunofluorescence for PECAM1 (red) and GFP (green) showing that Npr3CreERT2 activated by tamoxifen at P0 (iP0) and E17.5 (iE17.5) effectively drives GFP expression specifically in ventricular endocardium at P2 and P0, respectively. Quantitative analysis indicating 85-90% of ventricular endocardial cells (VEC) were marked by GFP at either stage, two days after tamoxifen indication. n=5 hearts for a and b. Data are presented as mean ± SEM and two-tailed unpaired Student’s t-test. Scale bar: 500 µm in a and b.
