Extended Data Fig. 6: p53 function inhibits lymphangiogensis in the absence of Ddx21. | Nature Cell Biology

Extended Data Fig. 6: p53 function inhibits lymphangiogensis in the absence of Ddx21.

From: The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Extended Data Fig. 6

(a) Confocal projections of a wild type, and p53zdf1, ddx21uq20bh, ddx21uq20bh;p53zdf1, vegfchu5055, vegfchu5055;p53zdf1 mutants at 5 dpf. Scale bars= 100μm. (b) Quantification of lymphatic endothelial cells (LECs) by scoring labelled nuclei present in lymphatics. p = 0.9506 for siblings (n = 29, ddx21uq20bh) versus p53zdf1 (n = 17, ddx21uq20bh). p < 0.0001 for ddx21uq20bh (n = 19) versus ddx21uq20bh;p53zdf1 (n = 5). p = 0.8551 for siblings (n = 11, vegfchu5055) versus p53zdf1 (n = 8, vegfchu5055). p = 0.8453 for vegfchu5055 (n = 20) versus vegfchu5055;p53zdf1 (n = 8). (c) Confocal projections of IF for endothelial nuclei (Tg(fli1a:nEGFP), green) and p53 (magenta) in a control and p53 morpholino-injected 48 hpf sibling and ddx21uq20bh mutant embryos. Arrow indicates a p53-positive PL. Scale bar= 50μm. (d) Left: Quantification of the number of p53 and GFP double positive lymphatic endothelial cells (LECs) and venous endothelial cells (VECs) in control siblings (n = 10) and ddx21uq20bh mutants (n = 10), and MO-p53-injected siblings (n = 8) and ddx21uq20bh mutants (n = 8). p = 0.0006 for control siblings versus ddx21uq20bh. p = 0.9982 for MO-p53-injected siblings versus ddx21uq20bh. p = 0.9907 for control siblings versus MO-p53-injected siblings. p = 0.0009 for control ddx21uq20bh mutants versus MO-p53-injected ddx21uq20bh mutants. Right: Quantification of the number of p53 positive gut cells in control siblings (n = 16) and ddx21uq20bh mutants (n = 24), and MO-p53-injected siblings (n = 5) and ddx21uq20bh mutants (n = 5). p = 0.001 for control siblings versus ddx21uq20bh. p > 0.9999 for MO-p53-injected siblings versus ddx21uq20bh. p = 0.4499 for control siblings versus MO-p53-injected siblings. p = 0.0138 for control ddx21uq20bh versus MO-p53-injected ddx21uq20bh. n=biologically independent samples (embryos) analysed. DA = dorsal aorta, PCV = posterior cardinal vein. Mean values + /-SEM shown. Two-tailed Mann Whitney test for (b). Ordinary one-way ANOVA test with Tukey’s multiple comparisons test for (d, LECs and VECs). Kruskal-Wallis test with Dunn’s multiple comparisons test for (d, gut cells).

Source data

Back to article page