Extended Data Fig. 7: Endothelial nucleolar Ddx21 is modulated by Vegfc-Flt4 signalling. | Nature Cell Biology

Extended Data Fig. 7: Endothelial nucleolar Ddx21 is modulated by Vegfc-Flt4 signalling.

From: The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Extended Data Fig. 7

(a) Confocal projections of IF for endothelial nuclei (Tg(fli1a:nEGFP), green) and Fibrillarin (magenta) in a 36 hpf sibling and a ddx21uq20bh mutant. Scale bars = 10μm. White brackets were cropped for Fig. 6l. (b) Confocal projections of 3 dpf control and vegfc-induced embryos on TgBAC(ddx21:ddx21-Citrine);Tg(prox1a:Kalt4,4xUAS:TagRFP);(10xUAS:vegfc) background. Images on the right show Ddx21-Citrine expression (grey) of images on the left. Scale bars= 50μm. (c) Confocal projections of control and MO-ccbe1-injected 40 hpf TgBAC(ddx21:ddx21-Citrine);Tg(lyve1b:DsRed2) embryos. White arrows show nucleolar Ddx21 expression in PCV ECs. Quantification in Fig. 7d (right). Scale bars= 20 μm. Images represent 7 animals (embryos) analyzed for both control and MO-ccbe1. DA = dorsal aorta, PCV = posterior cardinal vein.

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