Extended Data Fig. 4: FRAP experiments show a decreasing order of turnover for Sra1-AD, Sra1 and WCA*.
a, GFP fluorescence images of GFP-Sra1 transfected in Sra1/PIR121-KO B16 cells (upper). GFP-Sra1 time-lapse images during a FRAP experiment (bottom). The outlined area in the upper panel is shown at a higher magnification in the bottom panel. Scale bar, 5 µm. b, Analysis of GFP-Sra1 fluorescence recovery in FRAP experiments performed as in a (mean ± s.e.m. for cells) (n = 24 cells). The recovery is quantified from the outlined area in a bottom. c, d, Same as a,b for GFP-Sra1-WCA* (n = 25 cells). e, f, Same as a,b for GFP-Sra1-AD (n = 30 cells). Images in a, c, d, are representative of 2 independent experiments that yielded similar results. b, d, f, Data pooled from 2 independent experiments. Numerical source data are provided in Source data.
