Extended Data Fig. 1: Prototypic trajectories corresponding to the different diffusion modes: immobile, confined and free-diffusive.
a, Trajectories obtained from a sptPALM acquisition on mEos2-Nap1 in the lamellipodium of a spreading MEF (same cell as in Fig. 1a, 50 Hz, duration: 150 s). Trajectories are color-coded according to their diffusion modes: diffusive (gray), confined (yellow) and immobile (red) (top). b, Prototypic trajectories corresponding to distinct diffusion modes (immobile, confined and free-diffusive) highlighted in a. c, Illustration of the Mean Squared Displacement (MSD) computation from Δt to 4 x Δt using the equation in the inset, where xi and yi are the coordinates of the label position at time \(i \times {\Delta}t\). We defined the measured diffusion coefficient D as the slope of the affine regression line fitted to the n=1 to 4 values of the\(MSD\left( {n \times {\Delta}t} \right)\) (dotted line in the graph). d, Variation of the MSD over time for selected and highlighted trajectories in a and b. e, Variation of the MSD over time for all the trajectories of the sptPALM sequence with the same color-code as in a, mean ± s.e.m. for trajectories. Numerical source data are provided in Source data.
