Fig. 1: XRCC1 promotes the recovery of transcription following oxidative damage.
From: XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair
a, Representative images of RNAPI foci (RPA194) showing the levels of global transcription (EU pulse labelling) in WT and XRCC1−/− RPE-1 cells following mock treatment (NT) or at the indicated times after treatment with 250 μM H2O2 for 5 min. The cells were labelled with EU for 20 min before fixation. Scale bar, 10 μm; DAPI, 4,6-diamidino-2-phenylindole. b,c, Levels of global transcription (b; EU immunofluorescence) and RNAPI foci (c; RPA194) from the experiment shown in a. Data are the mean ± s.e.m. of three independent experiments. Statistical significance was determined using a two-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test (significantly different P values are indicated). d, Immunoblot of RNAPII hyperphosphorylation (hyper) in WT and XRCC1−/− RPE-1 cells following mock treatment or 2 h after treatment with 250 μM H2O2 for 5 min. A representative blot from one of three independent experiments is shown. The positions of the molecular mass markers are indicated; hypo, hypophosphorylation.
