Fig. 3: Toxic PARP1 activity suppresses transcriptional recovery in patient-derived fibroblasts with XRCC1 mutations and Xrcc1-deficient mouse cerebellar neurons.
From: XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair
a, Representative images of RNAPI foci (RPA194) showing the levels of global transcription (EU pulse labelling) in normal (1BR) and patient-derived fibroblasts with XRCC1 mutations (XD1) following mock treatment or at the indicated times after treatment with 60 μM H2O2 for 5 min. The cells were pulse labelled with EU as in Fig. 1. b, Levels of global transcription (EU immunofluorescence) from the experiment shown in a. Data are the mean ± s.e.m. of three independent experiments. Statistical significance was determined using a two-way ANOVA with Sidak’s multiple comparisons test (significantly different P values are indicated). c, Representative images of the global transcription levels (EU immunofluorescence) in WT and Xrcc1Nes-cre mouse cerebellar neurons (NeuN), pretreated with PARPi, following mock treatment or at the indicated times after treatment with 250 μM H2O2 for 5 min. Representative images from one of three independent experiments are shown. Scale bars, 10 μm.
