Extended Data Fig. 1: XRCC1 promotes the recovery of transcription following oxidative damage.
From: XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair
a, Immunoblot of RPA194 in WT and XRCC1−/− RPE-1 cells following mock treatment or 2 h after treatment with 250 μM H2O2 for 5 min. A representative image from one of three independent experiments is shown. b, Immunoblot of RNAPII hyperphosphorylation in WT and XRCC1−/− RPE-1 cells following mock treatment or 2 h after treatment with 250 μM H2O2 for 5 min. Where indicated, cells were pretreated with either 10 μM MG132 or 1 μM Bortezomib for 1 h before H2O2 treatment. A representative blot from one of three independent experiments is shown. c, RNAPI foci (RPA194) and levels of global transcription (EU immunofluorescence) in WT and XRCC1−/− RPE-1 cells pretreated for 1 h with 10μM MG132 or 1 μM Bortezomib following mock treatment or at 2 h after treatment with 250 μM H2O2 for 5 min. d and e, Quantification of the levels of global transcription (EU immunofluorescence) and RNAPI foci (RPA194) shown in (c). Data are means (±s.e.m.) of three independent experiments.
