Extended Data Fig. 7: Orthotopic transplantation of cancer organoids as a model to study gastric cancer. | Nature Cell Biology

Extended Data Fig. 7: Orthotopic transplantation of cancer organoids as a model to study gastric cancer.

From: A tumour-resident Lgr5+ stem-cell-like pool drives the establishment and progression of advanced gastric cancers

Extended Data Fig. 7

(a) E–Cadherin, Cdx2 and Tff2 IHC images for uninduced gastric organoids and (b) gastric cancer organoids (n = 3 biological replicates). (c) Whole mount images and H&E images for stomach, liver and lung 12 weeks post orthotopic transplantation with Cldn18–dTom organoids. IHC images for tdTomato, GFP and Ki67 in the stomach (n = 5 biological replicates). (d) IHC images for GFP, tdTomato, Ki67, Mapk, β–catenin, E–Cadherin, Cdx2 and Tff2 in liver (top) and lung (bottom) metastases 12 weeks post orthotopic transplantation (n = 5 biological replicates). Scale bars: panels A and B 50μm, remaining panels 100 μm.

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