Extended Data Fig. 9: Characterising the Tumour Resident Lgr5+ stem–like cell regeneration.
(a) FACS gating for sorting GFP+ and GFP- epithelial cells (EpCam+) from untreated tumours (left), DT-treated tumours (middle) and wild-type unstained controls (right). (b) Relative expression of Lgr5, GFP, Axin2, Troy, Sox2 and Mist1 in the GFP– cells from normal vs tumour corpus epithelium (Normal epithelium: n = 3, tumour epithelium n = 6 biological replicate). Statistical significance was determined by unpaired t-test; ***P = 0.0004 (Lgr5), ***P = 0.0002 (GFP), ***P = 0.0005 (Sox2), ***P = 0.0001 (Mist1). (c) Relative expression of Axin2, Troy, Sox2 and Mist1 in the GFP– cells from untreated vs DT-treated tumour epithelium (untreated tumours n = 6, DT-treated tumour n = 14 biological replicates, except Mist1: DT-treated tumour n = 10 biological replicates). Statistical significance was determined by unpaired t-test; ****P = < 0.0001 (Troy), *P = 0.0116 (Sox2), **P = 0.0089 (Mist1). Graphs are presented as mean ± s.d.
