Extended Data Fig. 7: Biogenesis of PDH-containing MDVs depends on DRP1.
From: MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control
a-c) SDS-PAGE analysis of DNM2 and DRP1 KO COS-7 cell lines (a), DRP1 KO COS-7 cells stably expressing human DRP1 (b) and DRP1 KO HeLa cells (c). * = unspecific cross reaction. d) Quantification of TOM+-MDVs in HeLa WT and two DRP1 KO clones. Counted MDVs are positive for TOMM40 and negative for PDH and MRPL12. Single values and means of three experiments are depicted in orange, cyan and grey. Means were used to calculate averages (horizontal bars), standard deviations (error bars), and p-values (two-tailed unpaired Student’s t-test: ** = p ≤ 0.01, n = 3 biologically independent experiments). e-g) Quantifications of TOM+-MDVs (e), double-positive TOM+/PDH+-MDVs (f) as well as classical PDH+-MDVs (g) in WT, DRP1 KO and DRP1 KO COS-7 cells rescued with human DRP1 grown under galactose conditions (5 days). Counted MDVs are negative for PDH and MRPL12 (e), MRPL12 (f), or TOMM40 and MRPL12 (g). Single values and means of three experiments are depicted in orange, cyan and grey. Means were used to calculate averages (horizontal bars), standard deviations (error bars), and p-values (two-tailed unpaired Student’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, n = 3 biologically independent experiments). Unprocessed blots and numerical source data including exact p values and test statistics are available in source data.
