Extended Data Fig. 2: Discrete structure-based characterization of RI2FL performance. | Nature Cell Biology

Extended Data Fig. 2: Discrete structure-based characterization of RI2FL performance.

From: Label-free multiplexed microtomography of endogenous subcellular dynamics using generalizable deep learning

Extended Data Fig. 2

Discrete subcellular structures, specifically lipid droplets for acceptable segmentation, were detected and matched in the inferred and ground-truth FL data in the held-out dataset. (a) Schematic summarizing the segmentation procedure (see Methods for details). (b) Matched versus unmatched lipid droplets could be well-defined based on inter-centroid distance and overlap fraction. (c) Distribution of inter-centroid distance in the matched lipid droplets. (d) Correlation between measured and inferred intensity averaged over the lipid droplet segments. The black line represents the least-squares linear regression fit of the data. N = 282 lipid droplets. r, Pearson’s correlation coefficient (calculated using the definition). Numerical source data is provided in source data.

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