Extended Data Fig. 3: PKCβII has a direct impact on ferroptosis sensitivity.
From: PKCβII phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis
An immunoblot showing the expression of PKCβII in HS578T (a), MDA-MB-468 (g), MCF7 (m) and BT474 (s) cells subjected to the indicated treatments. Data in a, g, m, s are representative of n = 3 biologically independent experiments. (b-e, h-k, n-q, t-w) Cell viability measurements in cells subjected to the indicated treatments. (f, l, r, x) Lipid peroxidation measurements in the indicated cells subjected to the indicated treatments for 20 h. f and l, Erastin, 10 μM; RSL3, 6 μM, Time, 24 h. r and x, Erastin, 15 μM; RSL3, 6 μM, Time, 24 h. b-e, h-k, n-q, t-w, error bars are mean ± s.d., n = 3 independent repeats. Statistical analysis was performed using a two-way ANOVA analysis. f, l, r, x, the mean ± s.d. of n = 3 independent experiments are shown. Statistical analysis was performed using an unpaired two-tailed t test.
