Extended Data Fig. 8: The ACSL4-T328D variant stably expressed in PKCβII knockout cells suffice to re-sensitize to ferroptosis.
From: PKCβII phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis
(a, f) An immunoblot showing the expression of ACSL4 in MDA-MB-231 (a) and HT1080 (f) cells subjected to the indicated treatments. (b-e, g-j) Cell viability measurements in cells subjected to the indicated treatments. (k,l) Lipid peroxidation (k) and cell death (l) measurements in cardiomyocytes cells subjected to the indicated treatments. (m) Endogenous ACSL4 was immunoprecipitated from cardiomyocytes cells subjected to the indicated treatments, followed by immunoblotting using an ACSL4 phospho-T328 antibody. Data in a, f, m, are representative of n = 3 biologically independent experiments. b-e, g-j, error bars are mean±s.d., n = 3 independent repeats. Statistical analysis was performed using a two-way ANOVA analysis. K,l, the mean±s.d. of n = 3 independent experiments are shown. Statistical analysis was performed using an unpaired two-tailed t test.
