Extended Data Fig. 5: Tumor cell-derived GABA excludes T cells from the tumor microenvironment.
From: Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression
a, Two-dimensional T-distributed stochastic neighbor embedding (t-SNE) analysis of RNA-seq data (n = 3 per group). b, Flow cytometry analysis of percentages of CD4+ T cells, CD8+ T cells, Treg, DC, CD103+ DC, and MDSC in subcutaneous LG1233 tumors as indicated conditions from C57BL/6 mice (n = 6 per group). c, Flow cytometry analysis of representative examples of CD4 and CD8 staining (gated live, TCRβ) in LLC-ova (top) and LG1233 (bottom) tumors stably expressing Gad1 shRNAs (sh1 and sh2) or NTC. d, Flow cytometry analysis of percentages of neutrophils, eosinophils, and macrophages in subcutaneous MC38 tumors as indicated conditions from C57BL/6 mice (n = 5 per group). e, MC38 cells stably expressing Gad1 shRNAs or NTC were subcutaneously injected into C57BL/6 mice (n = 5 per group). Intratumoral injection of saline or saline containing GABA was initiated on day 15. Average food consumption per mouse per day and mouse body weight on the indicated days were shown. n indicates the number of biological replicates. Data are presented as the mean ± SEM (b, d) and mean ± SD (e). P-values were calculated by two-tailed Student’s t test (b, d, e, left) and two-way ANOVA (e, right). *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant. Exact P values can be found in the Source Data.
