Extended Data Fig. 3: Cu-dependent LOX activity is not required for VEGF-induced EC proliferation.
From: Cysteine oxidation of copper transporter CTR1 drives VEGFR2 signalling and angiogenesis
A and D. HUVECs treated with Cu chelator, TTM (20 nM) for 24 hr were used to measure LOX activity in conditioned media (A) or cell proliferation measured by BrdU incorporation (D) after VEGF stimulation for 24 hr. B. HUVECs treated with Cu chelator TTM for 24 hr were stimulated with CuCl2 (25 µM) for 5 min, and lysates were used to immunoblotted (IB) with anti-p-MEK1/2 or p-ERK1/2 and their total proteins antibodies. C. HUVECs treated with specific LOX inhibitor, BAPN (100 µM) for 24 h were used to measure VEGF-induced cell proliferation as described. (n = 3 biologically independent experiments). A and B, two-tailed unpaired t-test. C and D, one-way ANOVA followed by Tukey’s multiple comparisons analysis, *p < 0.05, **p < 0.01, ***p < 0.001. NS = not significant. Data are mean ± SEM. Source numerical data and unprocessed blots are available in source data.
