Extended Data Fig. 1: Additional characterization of the monoastral mitotic spindle.
From: A monoastral mitotic spindle determines lineage fate and position in the mouse embryo

a, Additional examples of 8- to 16-cell stage mouse embryos displaying monoastral and anastral mitotic spindles. Dashed lines mark cortical microtubules. Data are from seven independent experiments. b, Light-scattering measurements in embryos injected with GFP mRNA at the 2-cell stage and imaged during the 8- to 16-cell stage. Quantification of GFP fluorescence along the axial (z) axis reveals no light scattering along the entire depth of a cell. Error bars represent s.e.m. Data are from three independent experiments. c, Monoastral spindles frequency is similar in different genetic backgrounds (p = 0.99 by Kolmogorov-Smirnov test). Scale bars, 5 µm in xyz.