Extended Data Fig. 2: RecT-like SSAP candidate screening.
From: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences
(a) Representative FACS plots of gating strategy for the current study. (b) Phylogenetic tree and amino acid alignment of representative RecT homologues along with the protein conserved domain annotated. (c) Screening RecT like SSAP candidates via metagenomics homologue mining and knock-in assay. The most active candidate is labelled as dCas9–SSAP. Data from 2 biologically independent experiments are shown.
