Extended Data Fig. 3: Limits of cell cycle marker single cell distributions and multi-channel gating interpretation in tissues.
From: Temporal and spatial topography of cell proliferation in cancer
a. Representative single-channel cell cycle marker images from CyCIF imaging with E-cadherin from tissue in Fig. 3a (scale bar 100 µm, example from 3 biological independent specimens). b. Single-channel distributions and two-dimensional scatter plots of a subset of cell cycle markers from HER2 positive breast cancer case #2 in Fig. 1c,e (n = 1,000 epithelial/tumour cells, log2 signal per cell prior to normalization). c. Single channel distributions for cell lineage, proliferation, and cell cycle markers from three HER2 + breast cancer samples (kernel smoothing (KS) density approximation) used in Fig. 1c,e. For proliferation and cell cycle markers only epithelial/tumour cells were used. d. Upset plot of three HER2 + breast cancer samples (n = 0.52 million cells) from Fig. 1c, e showing frequency of cell cycle marker positivity and their combinations sorted by frequency.
