Extended Data Fig. 7: Cell cycle dynamics from ccD-CMD from human cancer tissues.
From: Temporal and spatial topography of cell proliferation in cancer
a. Scatter plot of CFD versus IOV (for each ROI from Fig. 4f). b. K-means clustering of cell cycle markers from selected ROIs used in Fig. 4g (n = 3600 cells per ROI, k = 15 clusters). Left, single-cell clustering with ROI annotation (log2 normalized FAU). Middle, cluster median. Right, ROI composition for each cluster. c. Scanned image of hematoxylin and eosin (H&E) stained section from three HER2 positive breast tissues with pathology annotations. d. Composite CyCIF images of tissues from panel c (green CD45, red Vimentin, white E-Cadherin, scale bar 2 mm). e. Inter-Octile Variation IOV, f. Circle Fit Distance (CFD), and g. MPI + 1 fraction for each ROI noted in panel d.
