Extended Data Fig. 3: STX17 is recycled from autolysosomes.
From: Recycling of autophagosomal components from autolysosomes by the recycler complex
a, STX17 is recycled from autolysosomes. U2OS cells stably expressing GFP-STX17 TM, BFP-LC3 were starved for 2 h with EBSS and stained with LysoTracker. Time-lapse images were taken. Selected frames were shown as indicated time points. Scale bar, 1 μm. b-c, The recycling events and recycling frequency on autolysosomes were analyzed. Data are presented as mean values ± s.e.m., (n = 41 biologically independent experiments). d-f, Hela cells, HepG2 cells and COS-7 cells were transfected with GFP-STX17-TM. Twenty-four hours after transfection, cells stained with LysoTracker were starved and time-lapse images were taken. Selected frames were shown as indicated time points. Scale bar, 500 nm. Source numerical data are available in source data.
