Extended Data Fig. 1: GFP-STX17-TM fails to get to lysosomes in FIP200 knock-out cells and ATG9A knock-out cells.
From: Recycling of autophagosomal components from autolysosomes by the recycler complex
a and c, The delivery of STX17 to autolysosomes was blocked in FIP200-KO cells. Wild-type or FIP200-KO MEF cells stably expressing GFP-STX17-TM were starved with EBSS for 2 hours in a or treated with bafilomycin A1 (100 nM) for another 3 hours after 2 hours EBSS starvation in c. Cells were stained with antibodies against GFP and LAMP1. Scale bar, 5 μm. Inset scale bar, 2 μm. b and d, Images from a and c were analyzed, data are means ± s.d. (n = 3, 50 cells from 3 independent experiments were quantified). Unpaired two-tailed t-test. e and g, The delivery of STX17 to autolysosomes was blocked in ATG9-KO cells. Wild-type or ATG9-KO Hela cells stably expressing GFP-STX17-TM were starved with EBSS for 2 hours in e or treated with bafilomycin A1 (100 nM) for another 3 hours after 2 hours EBSS starvation in g. Then cells were stained with antibodies against GFP and LAMP1. Scale bar, 5 μm. Inset scale bar, 2 μm. f and h, Images e and g were analyzed. Data are means ± s.d. (n = 3, 50 cells from 3 independent experiments were quantified). Unpaired two-tailed t-test. Source numerical data are available in source data.
