Extended Data Fig. 10: In vivo single crRNA activation by hyperdCas12a.
From: Multiplexed genome regulation in vivo with hyper-efficient Cas12a
a-c, Constructs containing hyperdCas12a and single crRNA to Sox2 (a), Klf4 (b) or Oct4 (c) for in vivo electroporation in post-natal mouse retina and representative immunofluorescence images. CAG-GFP is used to mark the electroporated patch. Scale bar, 50 µm. Magnified views of the regions in the yellow boxes are shown in Fig. 6a. d, Quantification of percentages of Oct4+, Sox2+ or Klf4+ cells among HA + cells in INL. Bar graph shows the mean of 3 independent experiments, and each data point represents value of an independent experiment. e, Immunofluorescence images of in vivo electroporation in mouse retina with hyperdCas12a with non-targeting LacZ crRNA. Scale bar, 50 µm.
