Extended Data Fig. 3: Improved gene editing by hyperCas12a.
From: Multiplexed genome regulation in vivo with hyper-efficient Cas12a
a. Nuclease-active WT Cas12a versus hyperCas12a were co-transfected with crGFP into HEK293T cells stably expressing GFP driven by SV40 promoter. b. Representative flow cytometry histogram showing threshold for mCherry+ cells. Analysis of mCherry+ cells are shown in Fig. 2d, while bulk cells (without sorting) were used for indel analysis (panels c-d). c. Indel activity at each nucleotide position, shown as percentage of total reads with a deletion at the position. The PAM is highlighted in pink. d, Indel patterns and corresponding ratios in total reads detected by deep sequencing as analysed by CRISPResso252.
