Extended Data Fig. 4: Mutations of D and E residues in CycT1 IDR into A does not diminish MNNG-induced CycT1 PARylation; PARG treatment generates a homogenous pool of MARylated CycT1. | Nature Cell Biology

Extended Data Fig. 4: Mutations of D and E residues in CycT1 IDR into A does not diminish MNNG-induced CycT1 PARylation; PARG treatment generates a homogenous pool of MARylated CycT1.

From: Poly(ADP-ribosylation) of P-TEFb by PARP1 disrupts phase separation to inhibit global transcription after DNA damage

Extended Data Fig. 4

a, HeLa cells expressing the indicated CycT1-F proteins were untreated or treated with MNNG. The anti-Flag immunoprecipitates (IP) from whole cell extracts (WCE) were analyzed by Western blotting (WB). b, The immobilized af1521-Strep was incubated with WCE of HeLa cells untreated or treated with MNNG or/and PARG as indicated. The input and bound proteins were analyzed by WB. c, A schematic representation displaying PARG’s hydrolysis activity toward the PAR chain to leave behind a mono-ADP-ribose unit of 541.06 Dalton (Da). d, The Af1521-enriched CycT1-IDRL17A was untreated or treated with PARG in vitro and then analyzed by WB. All Western blots are representative of three independent experiments. Gel source data are available online.

Source data

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