Extended Data Fig. 5: Identification of CycT1 PARylation sites by ETD LC-MS/MS and mutation of CycT1 PARylation sites doesn’t affect the binding to PAR and P-TEFb partners.
a, The electron transfer dissociation (ETD) tandem mass spectra of the identified ADP-ribosylated peptides of CycT1, with c-ions and z-ions marked in red and blue, respectively. b, The sequence of the CycT1 IDRL region (aa 401-650) with the 12 identified PARylation sites highlighted in red and the remaining serine residues labeled in blue. c, Purified and immobilized WT or mutant CycT1-F was incubated with autoPARylated PARP1. The input and pull-down proteins were analyzed by Dot blotting (DB) or Western blotting (WB). d, Nuclear extracts (NE) of HeLa cells expressing WT or mutant CycT1-F proteins and anti-Flag immunoprecipitates (IP) from NE were analyzed by WB for the indicated proteins. All Western blots are representative of three independent experiments. Gel source data are available online.
