Extended Data Fig. 2: AluYb8 qPCR reveals that DTCs travel to and persist within multiple mouse SkMs following intracardiac injection of breast cancer cells.
From: Unchecked oxidative stress in skeletal muscle prevents outgrowth of disseminated tumour cells
a) Schematic of mouse study to examine frequency of DTCs across organ site at early and late timepoints. b) AluYb8 amplification (fold change) at Day 3 post ic injection. n = 11 inoculated and 3 uninoculated mice. Lung, brain and TA, P < 0.0001 when multiple unpaired two-tailed t-tests, followed by the Holm-Sidak method, were run for comparison of tumour-bearing tissues to uninoculated controls. c) Representative IF images of matched brain, lung, SkM and bone marrow (BoMa) with DTCs at the early timepoint. Scale bar: 100 μm for lung, brain and BoMa. 10 μm for muscle. d) AluYb8 amplification (fold change) at Week 7. n = 11 inoculated and 3 uninoculated mice. Lung, brain, liver, BoMa and TA, **** P < 0.0001 when multiple unpaired two-tailed t-tests, followed by Holm-Sidak method, were run for comparison of tumour-bearing tissues to uninoculated controls. e) Representative IF images of matched brain, lung, SkM and BoMa with DTCs at the late timepoint. Scale bar: 100 μm for lung, brain and BoMa; 10 μm for muscle. f) Representative IF images of DTCs or clusters of tumour cells in the brain, lung or SkM. Ki67+ marks proliferating cells. Scale bar: 100 μm for lung, brain and BoMa; 10 μm for muscle. n = 15 cells each. For c, e and g, centre line represents the mean, and error bars the standard error of the mean (s.e.m.).
