Extended Data Fig. 3: Metabolic comparison of SkM-metastatic 4T1 cells pre- and post-injection reveal that 4T1-SkM adapt to SkM.
From: Unchecked oxidative stress in skeletal muscle prevents outgrowth of disseminated tumour cells
a) PLS-DA score plot comparing 4T1-parental and 4T1-SkM cells in tissue culture against 4T1-SkM SkM metastases, 4T1-SkM lung metastases, healthy SkM and healthy lung. b) Fold change enrichment of GSH-related metabolites (defined by KEGG’s GSH metabolism set) for 4T1-SkM v. 4T1-parental in culture, SkM metastases v. 4T1-SkM in culture, and lung metastases v. 4T1-SkM in culture. c) Metabolite Set Enrichment of the metabolites that were 2-fold enriched in healthy SkM versus healthy lung. Over Representation Analysis used the hypergeometric test; one-tailed P-values were provided after adjusting for multiple testing. d) Table displaying the mean values for the GSH-related metabolites in healthy SkM and healthy lung, followed by the fold-change difference between the two. e) Dot-plot of the ratio of reduced to oxidized glutathione (GSH:GSSG) for 4T1-SkM and 4T1-parental in culture, healthy SkM and healthy lung, and SkM- and lung- metastases. n = 3 replicates for 4T1-parental and 4T1-SkM culture samples. n = 4 muscle- and 3 lung- metastases, 6 healthy muscle and 3 healthy lung. An one-way ANOVA, followed by uncorrected Fisher’s LSD, was performed where *** P = 0.0004 for 4T1-parental v 4T1-SkM, **** P < 0.0001 for healthy SkM v. lung, * P = 0.037 for SkM metastases v. lung metastases. For e, centre line represents the mean, and error bars the standard error of the mean (s.e.m.).
