Fig. 1: The WR domain limits NANOG solubility. | Nature Cell Biology

Fig. 1: The WR domain limits NANOG solubility.

From: NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Fig. 1

a, NANOG constructs. FL, full-length; NTD, N-terminal domain; DBD, DNA-binding domain; CTD, C-terminal domain; WR, tryptophan repeats domain. b, Solubility of the NANOG constructs, determined from the fraction of protein remaining in the supernatant after centrifugation. Data are presented as mean ± s.d. (n = 3 independent replicates; 6 μM total concentration for all constructs, except for 100 μM NTD). c, 2D NMR 15N-HSQC spectra of the 15N NANOG NTD (500 μM), showing limited peak dispersion in the 1H dimension. d, 1D 1H NMR spectra of the NANOG CTD at 10 μM (256 scans; ~10 min), showing the absence of strong NMR backbone amide and tryptophan side-chain peaks. au: arbitrary unit.

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