Extended Data Fig. 7: Representative Auto and Cross-Correlation FCCS measurements. | Nature Cell Biology

Extended Data Fig. 7: Representative Auto and Cross-Correlation FCCS measurements.

From: NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Extended Data Fig. 7

a-b, Auto and Cross-Correlation FCCS measurements of Gata6-DNA (AF488/AF647) with 0–875 nM NANOG WT (a) and W8A (b). Individual normalized auto and cross-correlation functions (measurements, symbols; FCCS fits and residuals, lines). Additional data are shown in Supplementary Information. c, Auto and Cross-Correlation FCCS measurements of Gata6-DNA and Oct4-DNA (AF488/AF647) with 250 nM NANOG WT (left panel) and W8A (right panel). (Left panel), Auto FCCS curves of Oct4-DNA AF647 (black squares), Gata6-DNA AF488 (magenta circles) and cross-correlation curve (blue triangles) in the presence of WT NANOG (250 nM). (Right panel), Auto FCCS curves of Oct4-DNA AF647 (black squares), Gata6-DNA AF488 (magenta circles) and cross-correlation curve (blue triangles) in the presence of mutant W8A NANOG (250 nM). Cross-correlation (blue line) is observed only with NANOG WT.

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