Extended Data Fig. 8: Stem Cell Pluripotency Assays. | Nature Cell Biology

Extended Data Fig. 8: Stem Cell Pluripotency Assays.

From: NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Extended Data Fig. 8

a, Fluorescence microscopy images of overexpressed GFP-tagged NANOG WT (left) and W8A mutant in ESCs. Similar results were observed with 2 biological replicates. The characteristic stem cell colonies are maintained in WT but not in W8A mutant. b, Lower magnification fluorescence microscopy images of overexpressed GFP-tagged NANOG WT (left) and W8A mutant (right) in ESCs showing widespread differentiation in the mutant. Similar results were observed with 2 biological replicates. c, Crystal violet staining (stain all cells) of ESCs. Similar results were observed with 2 biological replicates. d, Alkaline Phosphatase (AP) staining of ESC colonies with overexpressed GFP-tagged NANOG WT (left) and W8A mutant (right). More AP+ colonies were observed with WT than mutant. Similar results were observed with 3 biological replicates.

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