Fig. 5: NANOG PrD mutations alter DNA recognition in cells. | Nature Cell Biology

Fig. 5: NANOG PrD mutations alter DNA recognition in cells.

From: NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Fig. 5

a, Venn diagram summarizing the numbers of NANOG ChIP-seq peaks observed for WT or W8A expressed in HEK 293T cells. The binding sites are classified into three groups: WT only sites, WT/W8A shared sites and W8A only sites. b, Heatmaps of the normalized ChIP-seq reads of NANOG and H3K27ac on three groups (top to bottom): NANOG WT only, WT/W8A shared and W8A only sites, respectively. c, Top three non-redundant DNA motifs identified by HOMER from NANOG ChIP-seq data for the three classified groups. P values were generated by hypergeometric tests in HOMER. d, Cartoon diagram describing the possible modes (direct or indirect) of DNA recognition for the three groups. The shown ChIP-seq data were derived using two biologically independent replicates.

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