Extended Data Fig. 2: scRNAseq analysis of human NKX2.5eGFP/wt HPs in a chronic injury model of NHP heart slices.
a, Heatmap showing expression of the top 10 genes in each cluster defined as 0- early heart progenitors (Early HPs), 1- cardiac mesenchymal cells (CMCs), 2- ventricular cardiomyocytes (vCMs), 3- intermediate heart progenitors (IM HPs), 4- cardiac mesodermal cells (cMeso), 5- late heart progenitors (Late HPs), 6- proliferating heart progenitors (Prolif HPs). b, Heatmap of different blocks of DEGs along the pseudotime trajectory and representative genes in each cluster. Cardiac mesodermal precursors (CMPs, D-3), endothelial cell (EC) fate (D0 and D3) and CM fate (D21). Selected top biological process and canonical pathway terms related to corresponding DEGs. c, Heatmap showing the expression of genes related to contraction (gray and red) and metabolism (blue and green) in eGFP+ cells on D3 and D21 of ex-vivo co-culture compared to adult human LV-CMs (Wang et al., 2020). Expression levels are presented as a colour code. d, Progressive analysis of myofibril assembly, calcium handling and electrophysiology signatures by quantitative RT-PCR, n = 3 biological replicates. Expression levels are presented colour coded. For a-c, single cells have been isolated from 3 biological replicates. Data from all replicates are included. Exact p-values and numerical data are provided in Source Data Extended Data Fig. 2.
