Extended Data Fig. 4: scRNAseq analysis of human NKX2-5eGFP/wt HVPs and NHP cardiac cells after acute RFA heart injury.
a, Heatmap of top 50 genes in each cluster with representative genes indicated. 0. Early HVPs; 1. NHP CFs; 2. Activated HVPs; 3. Migrating HVPs; 4. NHP Mφ; 5. Proliferating HVPs; 6. Early vCMs. HVPs, human ventricular progenitors; NHP, non-human primate; CFs, cardiac fibroblasts; Mφ, macrophages; vCMs, ventricular cardiomyocytes. b, Representative GO terms upregulated in cluster 3 (migrating HVPs) compared to the other human clusters (0, 2, 5, 6). c, Violine plots of CXCL12 and its binding targets identified by italk analysis of scRNA sequencing of migrating HVPs, NHP CFs and NHP Mφ. d, Representative immunostainings of a-GFP, cardiac troponin T (cTNT) and DDR2 on D15 after injury at seeding site and RFA in the absence of CXCR4 blockage (AMD3100-) or in the presence of CXCR4 blockage (AMD3100+). Scale bars 25 µm. Statistical data are shown as mean ± SEM and individual data points, n = 3 biological replicates per condition, *** p < 0.001 (two-way ANOVA). For a-c single cells have been isolated from 3 biological replicates. Data from all replicates are included. Exact p-values and numerical data are provided in Source Data Extended Data Fig. 4.
